The dried leaves were stored in at room temperature for further use.The agar medium was allowed to solidify at room temperature.Then, mL of agar medium were added to each well of multidish and allowed to solidify. This method is widely used because it can determ ine the effect of volatile allelochemicals on the receiver plants very quickly. Multi well plastic dishes with six wells were used in this experiment.Approximately mg of the dried leaves sample were placed in the well at the corner six well multidishes.Four different distances from the sample were tested for allelopathic activity from leaves volatilization.In the control dish, sample well was left blank while other processes were repeated similar of each sample dish.Aluminium foils were wrapped around the multidishes to prevent interference of light.The radicle and hypocotyl length were measured and recorded thus compared to with control sample.The standard for working purpose was prepared freshly before use then the stock was stored in the chiller for further uses.The methanol extract was evaporated to dryness under vacuum at C using a rotary evaporator.The samples extract then centrifuged at rpm for minutes.This solution was used as test solution for HPTLC analysis.The loaded plate was kept in TLC twin trough developing chamber and the plate was developed in the respective mobile phase up to mm.The developed plate was dried using hot air to evaporate remaining solvents and sprayed with aluminium chloride reagent.Finally, the plate was fixed in scanner stage and scanned at nm.at the amount of mg. Blue and yellow color zone that were detected in UV after derivatization in the chromatogram suggested the presence of flavonoids. Among them, peaks and were identified as rutin and naringin.The experimental data were subjected to oneway analysis of variance at the level of significance.The statistical analysis was done using the SPSS software. This concentration dependent activity were also found in the previous research where it was reported that high concentration showed the highest inhibitory activity. Besides, the root tissue has higher permeability compared to the shoot tissue reported that the root development of receiver species was inhibited due to the ability of allelochemicals that can affect the genes which is responsible for cellular characterization of ground tissue and endoderm.Rutin and naringin are regarded as flavonoids, which are widely distributed in plants.This is due to ability of rutin in impairing respiration and ATP levels in embryogenic cells. The possible allelochemicals identified by HPTLC elucidated from methanolic extract were rutin and naringin.The authors wish to express their gratitude to agricultural officers from MADA for their invaluable assistance throughout the study.No use, distribution or reproduction is permitted which does not comply with these terms.Dish pack method: A new bioassay for volatile allelopathy.Chromatographic determ ination of allelochemicals. Screening the allelopathic activity of Bioscience Research, volume: Four aluminum plates ar ranged in a monopolarparallel configuration are utilized as electrodes in a ml EC reactor.The results reveal that a maximum COD removal efficiency of can be achieved by utilizing NSP power, while a value of only can be achieved by utilizing DC power.